nbp2 75624  (Novus Biologicals)

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Targeting the NF-κB p65-MMP28 axis: Wogonoside as a novel therapeutic agent for attenuating podocyte injury in diabetic nephropathy.

doi: 10.1016/j.phymed.2025.156406

Figure Lengend Snippet: Fig. 9. Antioxidant and anti-inflammatory effects via inhibition of the p65-MMP28 axis. (A) mRNA expression of Cat, Gsh-px, Tnf-α, Il-1β, and Il-6 in MPC-5 cells under NG and HG conditions with or without the p65 activator TNF-α (1 ng/ml) and wogonoside (40 μM) for 24 h. (B) mRNA expression of Cat, Gsh-px, Tnf-α, Il-1β, and Il-6 in MPC-5 cells under NG and HG conditions with or without the p65 inhibitor SC75741 (5 μM) and wogonoside (40 μM) for 24 h. (C) mRNA expression of Cat, Gsh-px, Tnf-α, Il-1β, and Il-6 in MPC-5 cells under NG and HG conditions with or without p65-siRNA followed by wogonoside (40 μM) treatment for 24 h. (D) mRNA expression of Cat, Gsh-px, Tnf-α, Il-1β, and Il-6 in MPC-5 cells under NG and HG conditions with or without p65 overexpression plasmid followed by wogonoside (40 μM) treatment for 24 h. (E) mRNA expression of Cat, Gsh-px, Tnf-α, Il-1β, and Il-6 in MPC-5 cells under NG and HG conditions with or without p65 overexpression plasmid followed by wogonoside (40 μM) treatment for 24 h. (F, G)The protein expression of Podocin in MPC-5 cells under NG or HG conditions with or without p65 overexpression and Mmp28 knockdown for 24 h followed by wogonoside (40 μM) treatment for 24 h. (H) The relative luciferase activity of Mmp28 under different conditions, including NG, HG, p65 overexpression, and p65 K221A overexpression. (I) The binding of IgG or p65 to the Mmp28 gene promoter, presented as signals relative to input. Data are presented as mean ± SEM, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. ns, no significant difference.

Article Snippet: The primary antibodies used were Podocin (1:200, Novus, JB51–33, USA), SOD2 (1:250, Abcam, ab68155, UK), 4-HNE (1:100, Thermo Fisher Scientific, MA5–27,570, USA), TNF-α (1:800, Proteintech, 60,291–1-Ig, China), IL-1β (1:500, Proteintech, 16,806–1-AP, China), and MMP28 (1:200, Proteintech, 18,237–1-AP, China).

Techniques: Inhibition, Expressing, Over Expression, Plasmid Preparation, Knockdown, Luciferase, Activity Assay, Binding Assay

Journal: Cell reports

Article Title: Deletion of STAT3 from Foxd1 cell population protects mice from kidney fibrosis by inhibiting pericytes trans-differentiation and migration

doi: 10.1016/j.celrep.2022.110473

Figure Lengend Snippet: KEY RESOURCES TABLE Primary and secondary antibodies

Article Snippet: Anti-Podocin , Novus Biologicals , JB51–33 , NBP2–75624.

Techniques: